Double-beam autocompensation for fluorescence polarization measurements in flow cytometry
نویسندگان
چکیده
منابع مشابه
Polarization of scatter and fluorescence signals in flow cytometry.
BACKGROUND The pulses of light scatter and fluorescence measured in flow cytometers exhibit varying degrees of polarization. Flow cytometers are heterogeneously sensitive to this polarization, depending on the light source(s), the optical layout, and the types of mirrors and filters used. Therefore, fluorescence polarization can affect apparent intensity ratios between particles and interfere w...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1985
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(85)83956-4